Journal: Molecular Biology Reports

Article Title: Regenerated silica-based RNA purification columns to address the short supply of RNA purification kits for COVID-19 diagnosis

doi: 10.1007/s11033-021-06688-0

Figure Lengend Snippet: Regenerated Viral-RNA purification kit columns have a comparable capacity for SARS-CoV2 RNA purification as the fresh columns. Bars represent the mean ± standard deviation Ct values for each SARS-CoV2 RT-qPCR target genes RdRp, E, N, and IC, for swab samples with Ct value ≤ 25 ( A ), Ct value = 25–35 ( B ), and Ct value = 36–40 ( C ). Each dot represents one sample. Pairwise comparisons of mean Ct values for each SARS-CoV2 target gene were done using a two-tailed paired Student’s t-test, with a confidence level of 95%. For low Ct value samples p value for RdRp, E and N genes were 0.18, 0.59 and 0.75 respectively, for moderate Ct value samples p value for RdRp, E and N genes were 0.57, 0.65 and 0.47 respectively, and for high Ct value samples p value for RdRp, E and N genes were 0.84, 0.85 and 0.86 respectively

Article Snippet: In our study, the method of regeneration and re-use of used RNA-purification columns for COVID-19 diagnosis was also extended for RNA-binding silica columns from two commercial sources (HiPurATM Viral RNA Purification Kit, HIMEDIA, and PureLink RNA Mini Kit, Invitrogen, Thermo).

Techniques: Purification, Standard Deviation, Quantitative RT-PCR, Two Tailed Test